| Introduction |
| Research |
| Staff Members |
| Employment |
| Contacting Us |
| Sandia Home |
| Links |
Rapid Deduction of Stress Response Pathways
in
Metal/Radionuclide Reducing Bacteria
As a part of the Virtual Institute for Microbial Stress and Survival, this project involves the characterization of regulatory networks in certain microorganisms as a part of U.S. Department of Energy’s 350 + cleanup projects for metal and radionuclide contaminated sites. We are a part of a Genomes-To-Life project funded by Department of Energy headed by Prof. Adam Arkin from LBNL and UC Berkeley. Other core research teams in the project can be found at the Virtual Institute for Microbial Stress and Survival. Currently we are closely examining three bacteria known for their ability to reduce metals:
Genome sequences of these microorganisms can be found at The Institute for Genomic Research. Efforts are currently under way to understand the regulatory networks in these anaerobic bacteria under a variety of stress conditions including heat, pH, oxygen to name a few. To this end a combination of transcriptomics, proteomics and bioinformatics approaches are being applied to identify genes involved in stress response pathways.
At Sandia National Laboratories, Livermore we
are interested in looking at protein
machines as well as protein expression levels
during exposure to stress conditions in
these organisms. Protein expression levels can
be studied using a variety of new
techniques that have spawned in the last few
years. Proteins rarely act solo. They
assemble into larger multi-component complexes
or molecular machines, to carry out
functions such as cell-to-cell signaling, transport
across membranes, protein synthesis
and everything else in a cell and between cells.
To complement our proteomics studies
on protein expression levels we are working on
novel technologies that will enable
systematic identification, characterization,
and eventually, understanding of the
majority of the multi-protein molecular machines
in these organisms. Bait proteins
with specific affinity tags can be used to "pull
out" associated proteins in the
complex. High throughput techniques to isolate
a large number of protein complexes
are currently under development.
List of investigators:
Anup Singh (Project Leader)
Contact Information
Dr. Anup Singh
Biosystems Research Department
Sandia National Laboratories
MS 9292, PO Box 969
7011 East Avenue
Livermore, CA 94551-0969
aksingh@sandia.gov
phone: 925-294-2949
fax: 925-294-3020